Hotspot

atpF-atpH

LSC · 13134-13323 · 190 bp

Back to Thelypteris

Pi 0.0351

MarkerSeek score 65.30

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 190
variable_sites: 10
pi: 0.0351
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	GAAAATTGCGATGCGGACAA	GCTTCGTCATTTGCCAATCC	45.0	50.0	58.0	58.1	255-260 bp	1.0	82.2
atpF-atpH_p2	GAAAATTGCGATGCGGACAA	GCTTTCACGGAAGCTTTGAC	45.0	50.0	58.0	58.0	300-305 bp	1.0	82.1
atpF-atpH_p3	GAAAATTGCGATGCGGACAA	GAAGGCAAGACACGAGGTAC	45.0	55.0	58.0	58.3	336-341 bp	1.0	79.9
atpF-atpH_p4	GAAAATTGCGATGCGGACAA	GGCAAGACACGAGGTACTTC	45.0	55.0	58.0	58.3	333-338 bp	1.0	79.9
atpF-atpH_p5	GAAAATTGCGATGCGGACAA	AGAAGGCAAGACACGAGGTA	45.0	50.0	58.0	58.4	337-342 bp	1.0	79.2