Hotspot

ycf1

SSC · 124258-129765 · 5508 bp

Back to Thamnosma

Pi 0.0135

MarkerSeek score 60.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5508
variable_sites: 74
pi: 0.0135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCGTATTCGCCCAGTTCTTG	CAGAAACCCCAGAAACGGAA	50.0	50.0	58.0	58.0	1150-1156 bp	1.0	82.0
ycf1_p2	GTCGTATTCGCCCAGTTCTT	CAGAAACCCCAGAAACGGAA	50.0	50.0	58.0	58.0	1151-1157 bp	1.0	82.0
ycf1_p3	TCGTATTCGCCCAGTTCTTG	GGAACAAGAGGTATCTGCCG	50.0	55.0	58.0	58.1	1080-1086 bp	1.0	81.2
ycf1_p4	GTCGTATTCGCCCAGTTCTT	GGAACAAGAGGTATCTGCCG	50.0	55.0	58.0	58.1	1081-1087 bp	1.0	81.2
ycf1_p5	TCGTATTCGCCCAGTTCTTG	AAAGAAACCCCAGAAACCCC	50.0	50.0	58.0	57.9	1160 bp	0.5	59.2