Hotspot

rpoC1

LSC · 20955-23799 · 2845 bp

Back to Thamnosma

Pi 0.0088

MarkerSeek score 47.10

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2845
variable_sites: 25
pi: 0.0088
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	CCCAAAGGGCCCTAGAATTC	ACGATCTTTGGCTTTGGAACT	55.0	42.9	57.9	58.1	2988 bp	0.5	50.3
rpoC1_p2	CCCAAAGGGCCCTAGAATTC	TCGAGAACTACGATCTTTGGC	55.0	47.6	57.9	57.9	2997 bp	0.5	50.2
rpoC1_p3	CCCAAAGGGCCCTAGAATTC	CGAGAACTACGATCTTTGGCT	55.0	47.6	57.9	57.8	2996 bp	0.5	50.2
rpoC1_p4	CCCAAAGGGCCCTAGAATTC	CGATCTTTGGCTTTGGAACTG	55.0	47.6	57.9	57.8	2987 bp	0.5	49.8
rpoC1_p5	CCCAAAGGGCCCTAGAATTC	ACGATCTTTGGCTTTGGAAC	55.0	45.0	57.9	56.6	2988 bp	0.5	48.4