Hotspot

ndhD-psaC

SSC · 117598-117767 · 170 bp

Back to Thamnosma

Pi 0.0414

MarkerSeek score 70.20

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 170
variable_sites: 7
pi: 0.0414
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	CTGCGGCTATCGGAAAAACT	AAACAACTCGAAGCATGGGT	50.0	45.0	58.6	58.0	254-275 bp	1.0	77.2
ndhD-psaC_p2	CTGCGGCTATCGGAAAAACT	GGCATGAAACAACTCGAAGC	50.0	50.0	58.6	58.0	260-281 bp	1.0	77.1
ndhD-psaC_p3	TGCGGCTATCGGAAAAACTA	AAACAACTCGAAGCATGGGT	45.0	45.0	57.3	58.0	253-274 bp	1.0	76.9
ndhD-psaC_p4	TGCGGCTATCGGAAAAACTA	GGCATGAAACAACTCGAAGC	45.0	50.0	57.3	58.0	259-280 bp	1.0	76.7
ndhD-psaC_p5	AAATGAACCTGCGGCTATCG	AAACAACTCGAAGCATGGGT	50.0	45.0	58.7	58.0	262-283 bp	1.0	76.6