Hotspot

ndhF-rpl32

SSC · 112999-113818 · 820 bp

Back to Thamnosma

Pi 0.0337

MarkerSeek score 66.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 820
variable_sites: 23
pi: 0.0337
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ATACGGCGAAGATGCTTTGT	CGTGGAGTGATGGGGAAAAT	45.0	50.0	58.0	57.9	1137-1363 bp	1.0	85.0
ndhF-rpl32_p2	GAAGATGCTTTGTTGCGGTC	CGTGGAGTGATGGGGAAAAT	50.0	50.0	58.0	57.9	1130-1356 bp	1.0	84.9
ndhF-rpl32_p3	TGTTGCGGTCGGAAAAAGTA	CGTGGAGTGATGGGGAAAAT	45.0	50.0	58.0	57.9	1120-1346 bp	1.0	84.7
ndhF-rpl32_p4	ATACGGCGAAGATGCTTTGT	AACGAAAAAGCCTTCAACGC	45.0	45.0	58.0	58.2	901-1037 bp	1.0	84.7
ndhF-rpl32_p5	GAAGATGCTTTGTTGCGGTC	AACGAAAAAGCCTTCAACGC	50.0	45.0	58.0	58.2	894-1030 bp	1.0	84.7