Hotspot

ndhK-ndhC

LSC · 50527-50584 · 58 bp

Back to Thamnosma

Pi 0.0357

MarkerSeek score 72.70

Primer available yes

Label ndhK-ndhC

Feature Metadata

label_name: ndhK-ndhC
feature_type: igs
region: LSC
length_bp: 58
variable_sites: 2
pi: 0.0357
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK-ndhC_p1	CCATACAGAAGCGGCCATAA	TGGGCAATGAGTTTGGATGT	50.0	45.0	57.7	57.7	295-297 bp	1.0	76.6
ndhK-ndhC_p2	ACAGAAGCGGCCATAAACTG	TGGGCAATGAGTTTGGATGT	50.0	45.0	58.5	57.7	291-293 bp	1.0	74.3
ndhK-ndhC_p3	CCATACAGAAGCGGCCATAA	TTGGTTCAATTTACGCGTGG	50.0	45.0	57.7	57.3	222-224 bp	1.0	73.9
ndhK-ndhC_p4	CCATACAGAAGCGGCCATAA	TGGCGAAAAGGAGCATTAGA	50.0	45.0	57.7	57.2	205-207 bp	1.0	73.3
ndhK-ndhC_p5	CCATACAGAAGCGGCCATAA	GGATGTATTGGGGGTATCCG	50.0	55.0	57.7	57.2	281-283 bp	1.0	72.8