Hotspot

psaA-ycf3

LSC · 42837-43569 · 733 bp

Back to Thamnosma

Pi 0.00956

MarkerSeek score 64.40

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 733
variable_sites: 7
pi: 0.00956
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGAGAAATGACCCGGTTTGG	AGCTATTGCGCTTACTCCTG	50.0	50.0	58.1	57.8	905-911 bp	1.0	78.1
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	AGCTATTGCGCTTACTCCTG	50.0	50.0	57.9	57.8	886-892 bp	1.0	77.9
psaA-ycf3_p3	TGAGAAATGACCCGGTTTGG	TGGAAACAAGCTATTGCGCT	50.0	45.0	58.1	58.5	913-919 bp	1.0	76.2
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	TGGAAACAAGCTATTGCGCT	50.0	45.0	57.9	58.5	894-900 bp	1.0	76.1
psaA-ycf3_p5	TGAGAAATGACCCGGTTTGG	GGAGGCCTGGTTCAATCAAG	50.0	55.0	58.1	58.5	944-950 bp	1.0	75.7