Hotspot

rpoC2-rpoC1

LSC · 20789-20954 · 166 bp

Back to Thamnosma

Pi 0.0422

MarkerSeek score 76.70

Primer available yes

Label rpoC2-rpoC1

Feature Metadata

label_name: rpoC2-rpoC1
feature_type: igs
region: LSC
length_bp: 166
variable_sites: 7
pi: 0.0422
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2-rpoC1_p1	TATGCCATTCCGAAGTGCTC	CGAACTACCGTTGGCCATAT	50.0	50.0	58.0	57.8	354 bp	1.0	79.3
rpoC2-rpoC1_p2	GTGAAAGACCAGACTCACCC	CGAACTACCGTTGGCCATAT	55.0	50.0	57.8	57.8	283 bp	1.0	78.7
rpoC2-rpoC1_p3	TATGCCATTCCGAAGTGCTC	ATACAAGGGTTTTGTCGGGC	50.0	50.0	58.0	58.5	306 bp	1.0	77.8
rpoC2-rpoC1_p4	GCCATTCCGAAGTGCTCTAT	CGAACTACCGTTGGCCATAT	50.0	50.0	57.8	57.8	351 bp	1.0	77.7
rpoC2-rpoC1_p5	AGACTCACCCGTTCTGTCAT	CGAACTACCGTTGGCCATAT	50.0	50.0	58.4	57.8	273 bp	1.0	77.2