Hotspot

atpF

LSC · 35340-36733 · 1394 bp

Back to Thamnocalamus

Pi 0.00431

MarkerSeek score 56.10

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1394
variable_sites: 6
pi: 0.00431
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	AAAAAGGGCGAGCGAAGTAA	TTCGGAAGCAAGGGGAAAAA	45.0	45.0	58.1	57.9	1534-1556 bp	1.0	80.0
atpF_p2	AAAAAGGGCGAGCGAAGTAA	TTTCGGAAGCAAGGGGAAAA	45.0	45.0	58.1	57.9	1535-1557 bp	1.0	80.0
atpF_p3	AAAAAGGGCGAGCGAAGTAA	TTTTCGGAAGCAAGGGGAAA	45.0	45.0	58.1	57.9	1536-1558 bp	1.0	80.0
atpF_p4	AAAAAGGGCGAGCGAAGTAA	TTTTTCGGAAGCAAGGGGAA	45.0	45.0	58.1	57.9	1537-1559 bp	1.0	80.0
atpF_p5	AAAAAGGGCGAGCGAAGTAA	TCGAAGGGTTGCCATTAGTG	45.0	50.0	58.1	57.9	1570-1591 bp	1.0	79.7