Hotspot

rps12-clpP

LSC · 70209-70351 · 143 bp

Back to Thamnocalamus

Pi 0.021

MarkerSeek score 64.60

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 143
variable_sites: 3
pi: 0.021
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	CACCCTAGCACATGTTCCTC	GCGATACTGATCCAGTGTGG	55.0	55.0	58.0	58.1	301 bp	1.0	79.4
rps12-clpP_p2	CACCCTAGCACATGTTCCTC	GATTGACGAGCACTGCGATA	55.0	50.0	58.0	58.2	315 bp	1.0	79.2
rps12-clpP_p3	CCTAGCACATGTTCCTCGAC	GCGATACTGATCCAGTGTGG	55.0	55.0	58.1	58.1	298 bp	1.0	79.1
rps12-clpP_p4	CCTAGCACATGTTCCTCGAC	GATTGACGAGCACTGCGATA	55.0	50.0	58.1	58.2	312 bp	1.0	78.9
rps12-clpP_p5	CACCCTAGCACATGTTCCTC	GAGCACTGCGATACTGATCC	55.0	55.0	58.0	58.2	308 bp	1.0	78.8