Hotspot

trnW-CAA-trnP-UGG

LSC · 66685-66814 · 130 bp

Back to Thamnocalamus

Pi 0.0233

MarkerSeek score 64.30

Primer available yes

Label trnW-CAA-trnP-UGG

Feature Metadata

label_name: trnW-CAA-trnP-UGG
feature_type: igs
region: LSC
length_bp: 130
variable_sites: 3
pi: 0.0233
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CAA-trnP-UGG_p1	GCGTTCTACCGAACTGAACT	CTTGGTAGCGCGTTTGTTTT	50.0	45.0	57.9	57.9	216-217 bp	1.0	80.1
trnW-CAA-trnP-UGG_p2	ACCTACGACATCGGGTTTTG	CTTGGTAGCGCGTTTGTTTT	50.0	45.0	57.9	57.9	243-244 bp	1.0	79.7
trnW-CAA-trnP-UGG_p3	GCGTTCTACCGAACTGAACT	TTGGTAGCGCGTTTGTTTTG	50.0	45.0	57.9	58.2	215-216 bp	1.0	79.6
trnW-CAA-trnP-UGG_p4	GCGTTCTACCGAACTGAACT	CGCGTTTGTTTTGGGTACAA	50.0	45.0	57.9	57.8	208-209 bp	1.0	79.5
trnW-CAA-trnP-UGG_p5	ACCTACGACATCGGGTTTTG	TTGGTAGCGCGTTTGTTTTG	50.0	45.0	57.9	58.2	242-243 bp	1.0	79.2