Hotspot

petA-psbJ

LSC · 63115-64102 · 988 bp

Back to Thamnocalamus

Pi 0.00506

MarkerSeek score 61.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 988
variable_sites: 5
pi: 0.00506
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTCGC	TCCTCTTTGGCTGATAGGTACT	50.0	45.5	57.9	58.0	1190 bp	1.0	63.9
petA-psbJ_p2	GGCCTTTTGTTCTTCTTCGC	CCTCTTTGGCTGATAGGTACTG	50.0	50.0	57.9	57.9	1189 bp	1.0	63.4
petA-psbJ_p3	GGCCTTTTGTTCTTCTTCGC	TTCCTCTTTGGCTGATAGGT	50.0	45.0	57.9	55.8	1191 bp	1.0	62.5
petA-psbJ_p4	GGCCTTTTGTTCTTCTTCGC	ACGGTTCATATTCTGGATTGGG	50.0	45.5	57.9	58.2	1125 bp	1.0	62.5
petA-psbJ_p5	GGCCTTTTGTTCTTCTTCGC	CGGTTCATATTCTGGATTGGGT	50.0	45.5	57.9	58.2	1124 bp	1.0	62.5