Hotspot

rbcL-psaI

LSC · 58492-59783 · 1292 bp

Back to Thamnocalamus

Pi 0.00233

MarkerSeek score 60.60

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 1292
variable_sites: 3
pi: 0.00233
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	AAATTTGAGTTCGAGCCGGT	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.1	58.0	1395-1401 bp	1.0	79.5
rbcL-psaI_p2	GCCGCAGCTTGTGAAATATG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.2	58.0	1425-1431 bp	1.0	79.0
rbcL-psaI_p3	AAATTTGAGTTCGAGCCGGT	AAGAAGCCATTGCAATTGCC	45.0	45.0	58.1	57.9	1401-1407 bp	1.0	78.9
rbcL-psaI_p4	GCCGCAGCTTGTGAAATATG	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.2	57.9	1431-1437 bp	1.0	78.4
rbcL-psaI_p5	CAAATTTGAGTTCGAGCCGG	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.8	58.0	1396-1402 bp	1.0	78.4