Hotspot

psaA-ycf3

LSC · 43874-44493 · 620 bp

Back to Thamnocalamus

Pi 0.00323

MarkerSeek score 60.60

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 620
variable_sites: 2
pi: 0.00323
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGTTCTTGAGAAATGGCCGG	TATAGCGCTTACTCCGGGAA	50.0	50.0	58.5	58.0	801 bp	1.0	76.4
psaA-ycf3_p2	TTTTACTTCTGGTTCCGGCG	TATAGCGCTTACTCCGGGAA	50.0	50.0	58.5	58.0	729 bp	1.0	76.2
psaA-ycf3_p3	TGTTCTTGAGAAATGGCCGG	GCGCTTACTCCGGGAAATTA	50.0	50.0	58.5	58.1	797 bp	1.0	76.0
psaA-ycf3_p4	TTTTACTTCTGGTTCCGGCG	GCGCTTACTCCGGGAAATTA	50.0	50.0	58.5	58.1	725 bp	1.0	75.9
psaA-ycf3_p5	GGTTTGGCCCATTCCTCAAA	TATAGCGCTTACTCCGGGAA	50.0	50.0	58.7	58.0	782 bp	1.0	74.9