Hotspot

trnC-GCA-rpoB

LSC · 20553-21735 · 1183 bp

Back to Thamnocalamus

Pi 0.00604
MarkerSeek score 52.10
Primer available yes
Label trnC-GCA-rpoB

Feature Metadata

label_name
trnC-GCA-rpoB
feature_type
igs
region
LSC
length_bp
1183
variable_sites
7
pi
0.00604
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-rpoB_p1 AAAGGATTTGCAGTCCCCTG TCCCTCATTTCCATTCCGGA 50.0 50.0 58.1 58.4 1227-1250 bp 1.0 78.2
trnC-GCA-rpoB_p2 AAAGGATTTGCAGTCCCCTG TTCCCTCATTTCCATTCCGG 50.0 50.0 58.1 57.6 1228-1251 bp 1.0 77.9
trnC-GCA-rpoB_p3 TTTATTGATCAGGCGGCACC TCCCTCATTTCCATTCCGGA 50.0 50.0 58.6 58.4 1265-1288 bp 1.0 73.8
trnC-GCA-rpoB_p4 TTTATTGATCAGGCGGCACC TTCCCTCATTTCCATTCCGG 50.0 50.0 58.6 57.6 1266-1289 bp 1.0 73.6
trnC-GCA-rpoB_p5 AAAGGATTTGCAGTCCCCTG ATTCCCTCATTTCCATTCCGG 50.0 47.6 58.1 58.1 1229-1252 bp 1.0 73.0