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psbM-petN

LSC · 18723-19490 · 768 bp

Back to Thamnocalamus

Pi 0.00398

MarkerSeek score 62.80

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 768
variable_sites: 3
pi: 0.00398
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TGGAAGTCAATATTCTCGCA	TAGTAAGTCTCGCTTGGGCT	40.0	50.0	54.5	58.2	941-955 bp	1.0	52.1
psbM-petN_p2	TGGAAGTCAATATTCTCGCA	GTAGTATGGGGGAGGAGTGG	40.0	60.0	54.5	58.3	885-899 bp	1.0	51.1
psbM-petN_p3	TGGAAGTCAATATTCTCGCA	TAGTATGGGGGAGGAGTGGA	40.0	55.0	54.5	58.4	884-898 bp	1.0	50.4
psbM-petN_p4	TGCTACTGCACTGTTTATTCT	TAGTAAGTCTCGCTTGGGCT	38.1	50.0	55.2	58.2	916-930 bp	1.0	50.2
psbM-petN_p5	TGCTACTGCACTGTTTATTCT	GTAGTATGGGGGAGGAGTGG	38.1	60.0	55.2	58.3	860-874 bp	1.0	49.3