Hotspot

psbI-trnS-GCU

LSC · 8265-8384 · 120 bp

Back to Thamnocalamus

Pi 0.0167

MarkerSeek score 62.10

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 120
variable_sites: 2
pi: 0.0167
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	TAATCCTGGGCGTGAGGAG	TAAAGCGGCGGATTGCTAAT	57.9	45.0	58.5	58.0	210 bp	1.0	68.6
psbI-trnS-GCU_p2	TAATCCTGGGCGTGAGGAG	CTTTCGATTTGGAACGGGGA	57.9	50.0	58.5	58.2	247 bp	1.0	67.3
psbI-trnS-GCU_p3	TAATCCTGGGCGTGAGGAG	TTTCGATTTGGAACGGGGAG	57.9	50.0	58.5	58.2	246 bp	1.0	67.3
psbI-trnS-GCU_p4	GTAATCCTGGGCGTGAGGA	TAAAGCGGCGGATTGCTAAT	57.9	45.0	58.8	58.0	211 bp	1.0	66.1
psbI-trnS-GCU_p5	TAATCCTGGGCGTGAGGAG	ATGGCTGAGTGGACTAAAGC	57.9	50.0	58.5	57.6	224 bp	1.0	65.6