Hotspot

matK-trnK-UUU

LSC · 3232-3930 · 699 bp

Back to Thamnocalamus

Pi 0.00579
MarkerSeek score 63.20
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
699
variable_sites
4
pi
0.00579
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 ACTCCTGAAAGAGAAGTGGGT CGTGCGGTGTAATTCCATTG 47.6 50.0 58.3 57.8 860-868 bp 1.0 69.3
matK-trnK-UUU_p2 ACTCCTGAAAGAGAAGTGGG CGTGCGGTGTAATTCCATTG 50.0 50.0 56.5 57.8 860-868 bp 1.0 67.5
matK-trnK-UUU_p3 CTCCTGAAAGAGAAGTGGGT CGTGCGGTGTAATTCCATTG 50.0 50.0 56.5 57.8 859-867 bp 1.0 67.5
matK-trnK-UUU_p4 ACTCCTGAAAGAGAAGTGGGT TGGGTTGCTAACTCAATGGT 47.6 45.0 58.3 57.0 801-809 bp 1.0 63.0
matK-trnK-UUU_p5 ACTCCTGAAAGAGAAGTGGGTA CGTGCGGTGTAATTCCATTG 45.5 50.0 58.1 57.8 860-868 bp 1.0 62.6