Hotspot

trnP-UGG-psaJ

LSC · 67417-67859 · 443 bp

Back to Thalictrum

Pi 0.0224

MarkerSeek score 64.30

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 443
variable_sites: 47
pi: 0.0224
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	GCCACGGATAGATATGTTTT	50.0	40.0	58.1	53.2	445-590 bp	1.0	51.5
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	GCCACGGATAGATATGTTTT	50.0	40.0	58.1	53.2	444-589 bp	1.0	51.5
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	GCCACGGATAGATATGTTTT	50.0	40.0	58.1	53.2	456-601 bp	1.0	51.3
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	GCCACGGATAGATATGTTTT	50.0	40.0	58.1	53.2	455-600 bp	1.0	51.3
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	GCCACGGATAGATATGTTTT	50.0	40.0	58.1	53.2	458-603 bp	1.0	51.2