Hotspot

psbA-trnK-UUU

LSC · 1313-1559 · 247 bp

Back to Thalictrum

Pi 0.0264

MarkerSeek score 73.10

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 247
variable_sites: 32
pi: 0.0264
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	AACACCGAACCATCCAATGT	ACTCCATCCGACTAGTTCCG	45.0	55.0	57.7	58.6	329-414 bp	1.0	83.3
psbA-trnK-UUU_p2	ACGGTTTTCAGTGCTGGTTA	ACTCCATCCGACTAGTTCCG	45.0	55.0	57.7	58.6	305-390 bp	1.0	83.3
psbA-trnK-UUU_p3	TCAAAACACCGAACCATCCA	ACTCCATCCGACTAGTTCCG	45.0	55.0	57.6	58.6	333-418 bp	1.0	82.7
psbA-trnK-UUU_p4	AACACCGAACCATCCAATGT	ACTAGTTCCGGGTTCGAGTC	45.0	55.0	57.7	58.8	319-404 bp	1.0	81.7
psbA-trnK-UUU_p5	AACACCGAACCATCCAATGT	GACTAGTTCCGGGTTCGAGT	45.0	55.0	57.7	58.8	320-405 bp	1.0	81.7