Hotspot

thiG

Genome · 110167-110976 · 810 bp

Back to Thalassiosira

Pi 0.117

MarkerSeek score 79.20

Primer available yes

Label thiG

Feature Metadata

label_name: thiG
feature_type: gene
region: Genome
length_bp: 810
variable_sites: 95
pi: 0.117
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
thiG_p1	GCACGCTTCTTCCTTACAGA	TGGTCGTAGGTTCAAGTCCT	50.0	50.0	57.9	58.0	1322-1343 bp	1.0	88.9
thiG_p2	CGTCCACTACGTCGTTCAAT	TGGTCGTAGGTTCAAGTCCT	50.0	50.0	58.0	58.0	1295 bp	0.5	59.7
thiG_p3	CGTCCACTACGTCGTTCAAT	ACGAACTAAAAACGTGGGCT	50.0	45.0	58.0	58.0	1656 bp	0.5	59.4
thiG_p4	CGTCCACTACGTCGTTCAAT	TTCAAGTCCTACCTGGGGAG	50.0	55.0	58.0	58.0	1285 bp	0.5	59.4
thiG_p5	AACCAAACGCCTACGGAAAT	TGGTCGTAGGTTCAAGTCCT	45.0	50.0	58.1	58.0	1186 bp	0.5	59.2