Hotspot

rpl2

Genome · 92654-93481 · 828 bp

Back to Thalassiosira

Pi 0.1

MarkerSeek score 79.20

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: Genome
length_bp: 828
variable_sites: 83
pi: 0.1
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	GGATCAAGCCGTTCTCCATT	GTCGAACTACGTGACCAAGT	50.0	50.0	58.0	57.6	1719 bp	0.5	56.3
rpl2_p2	TAGAGCTCGAGCAGGATCAA	GTCGAACTACGTGACCAAGT	50.0	50.0	57.9	57.6	1732 bp	0.5	56.2
rpl2_p3	AACACCGCTCATTTACCGAA	GTCGAACTACGTGACCAAGT	45.0	50.0	57.8	57.6	1136 bp	0.5	55.3
rpl2_p4	ACACCGCTCATTTACCGAAA	GTCGAACTACGTGACCAAGT	45.0	50.0	57.8	57.6	1135 bp	0.5	55.3
rpl2_p5	ATATCTGGGCTGGAAATCGC	GTCGAACTACGTGACCAAGT	50.0	50.0	57.8	57.6	1095 bp	0.5	55.2