Hotspot

rns-ycf89

Genome · 127988-128550 · 563 bp

Back to Thalassiosira

Pi 0.274

MarkerSeek score 82.70

Primer available yes

Label rns-ycf89

Feature Metadata

label_name: rns-ycf89
feature_type: igs
region: Genome
length_bp: 563
variable_sites: 143
pi: 0.274
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rns-ycf89_p1	CATTGCGGAAAATTCCCCAC	TGGTATTCAGAGCGTCCTGA	50.0	50.0	58.0	58.2	1087 bp	0.5	58.7
rns-ycf89_p2	TCTCGTACGACTTGCATGTG	TGGTATTCAGAGCGTCCTGA	50.0	50.0	58.0	58.2	805 bp	0.5	58.6
rns-ycf89_p3	TATTGCTCCGTCAGGCTTTC	TGGTATTCAGAGCGTCCTGA	50.0	50.0	58.0	58.2	1110 bp	0.5	58.6
rns-ycf89_p4	CAACCCATAGGCAGTCTTCC	TGGTATTCAGAGCGTCCTGA	55.0	50.0	58.0	58.2	1139 bp	0.5	58.4
rns-ycf89_p5	CTTGCATCCTCCGTCTTACC	TGGTATTCAGAGCGTCCTGA	55.0	50.0	58.1	58.2	1238 bp	0.5	58.3