Hotspot

rns-trnI-GAU

Genome · 69264-69322 · 59 bp

Back to Thalassiosira

Pi 0.103

MarkerSeek score 79.70

Primer available yes

Label rns-trnI-GAU

Feature Metadata

label_name: rns-trnI-GAU
feature_type: igs
region: Genome
length_bp: 59
variable_sites: 6
pi: 0.103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rns-trnI-GAU_p1	GACTGGGGTGAAGTCGTAAC	TTATCAGGGGTGCGCTCTAA	55.0	50.0	57.9	58.5	158-159 bp	1.0	78.9
rns-trnI-GAU_p2	GGAGGGTGCCTAAGGTAGAA	TTATCAGGGGTGCGCTCTAA	55.0	50.0	58.1	58.5	183-184 bp	1.0	78.0
rns-trnI-GAU_p3	GGGGTGAAGTCGTAACAAGG	TTATCAGGGGTGCGCTCTAA	55.0	50.0	58.2	58.5	154-155 bp	1.0	78.0
rns-trnI-GAU_p4	GACTGGGGTGAAGTCGTAAC	TGGGCCATTCTGGATTTGAA	55.0	45.0	57.9	57.4	193-194 bp	1.0	77.3
rns-trnI-GAU_p5	TCTAACCATTCGGAGGAGGG	TTATCAGGGGTGCGCTCTAA	55.0	50.0	58.2	58.5	197-198 bp	1.0	77.1