Hotspot

ycf89-rns

Genome · 67209-67771 · 563 bp

Back to Thalassiosira

Pi 0.274

MarkerSeek score 82.70

Primer available yes

Label ycf89-rns

Feature Metadata

label_name: ycf89-rns
feature_type: igs
region: Genome
length_bp: 563
variable_sites: 143
pi: 0.274
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf89-rns_p1	TGGTATTCAGAGCGTCCTGA	CATTGCGGAAAATTCCCCAC	50.0	50.0	58.2	58.0	1087 bp	0.5	58.7
ycf89-rns_p2	TGGTATTCAGAGCGTCCTGA	TCTCGTACGACTTGCATGTG	50.0	50.0	58.2	58.0	805 bp	0.5	58.6
ycf89-rns_p3	TGGTATTCAGAGCGTCCTGA	TATTGCTCCGTCAGGCTTTC	50.0	50.0	58.2	58.0	1110 bp	0.5	58.6
ycf89-rns_p4	TGGTATTCAGAGCGTCCTGA	CAACCCATAGGCAGTCTTCC	50.0	55.0	58.2	58.0	1139 bp	0.5	58.4
ycf89-rns_p5	TGGTATTCAGAGCGTCCTGA	CTTGCATCCTCCGTCTTACC	50.0	55.0	58.2	58.1	1238 bp	0.5	58.3