Hotspot

trnY-GUA-petG

Genome · 51174-51382 · 209 bp

Back to Thalassiosira

Pi 0.184

MarkerSeek score 79.60

Primer available yes

Label trnY-GUA-petG

Feature Metadata

label_name: trnY-GUA-petG
feature_type: igs
region: Genome
length_bp: 209
variable_sites: 37
pi: 0.184
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-petG_p1	GTAAATCCGCTGCGTTATGC	TCGTCGTGGAAATCAATTTGA	50.0	38.1	58.0	56.2	278-286 bp	1.0	67.7
trnY-GUA-petG_p2	AAATCCGCTGCGTTATGCTA	TCGTCGTGGAAATCAATTTGA	45.0	38.1	58.1	56.2	276-284 bp	1.0	67.5
trnY-GUA-petG_p3	TAAATCCGCTGCGTTATGCT	TCGTCGTGGAAATCAATTTGA	45.0	38.1	58.1	56.2	277-285 bp	1.0	67.5
trnY-GUA-petG_p4	AAGGGGGCGGATTGTAAATC	TCGTCGTGGAAATCAATTTGA	50.0	38.1	57.9	56.2	291-299 bp	1.0	67.2
trnY-GUA-petG_p5	TGCGTTATGCTACGTTGGTT	TCGTCGTGGAAATCAATTTGA	45.0	38.1	58.2	56.2	268-276 bp	1.0	66.3