Hotspot

psaM-rps14

Genome · 46772-46908 · 137 bp

Back to Thalassiosira

Pi 0.124

MarkerSeek score 79.30

Primer available yes

Label psaM-rps14

Feature Metadata

label_name: psaM-rps14
feature_type: igs
region: Genome
length_bp: 137
variable_sites: 16
pi: 0.124
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaM-rps14_p1	GCGACATAAACTTGAAAATCAGT	AGGGGTTACTAAATCAAGTTGGT	34.8	39.1	56.1	57.4	183-191 bp	1.0	50.3
psaM-rps14_p2	GCGACATAAACTTGAAAATCAGT	CAGGGGTTACTAAATCAAGTTGG	34.8	43.5	56.1	57.1	184-192 bp	1.0	50.2
psaM-rps14_p3	GCGACATAAACTTGAAAATCAGT	CCAGGGGTTACTAAATCAAGTTG	34.8	43.5	56.1	57.1	185-193 bp	1.0	50.2
psaM-rps14_p4	GCGACATAAACTTGAAAATCAGT	ACCAGGGGTTACTAAATCAAGT	34.8	40.9	56.1	56.7	186-194 bp	1.0	50.1
psaM-rps14_p5	GCGACATAAACTTGAAAATCAGT	ACCAGGGGTTACTAAATCAAGTT	34.8	39.1	56.1	57.4	186-194 bp	1.0	50.1