Hotspot

petA-chlI

Genome · 44542-45575 · 1034 bp

Back to Thalassiosira

Pi 0.374

MarkerSeek score 83.20

Primer available yes

Label petA-chlI

Feature Metadata

label_name: petA-chlI
feature_type: igs
region: Genome
length_bp: 1034
variable_sites: 360
pi: 0.374
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-chlI_p1	AGCAGAGCAACCGTTAAACA	GCAAAAGCACATGCAGCATA	45.0	45.0	58.0	58.0	1398 bp	0.5	59.7
petA-chlI_p2	AGCAGAGCAACCGTTAAACA	GGTATGCATGCCGAAATTCG	45.0	50.0	58.0	58.0	1662 bp	0.5	59.4
petA-chlI_p3	AGCAGAGCAACCGTTAAACA	CAGCATACAATGGTCGGGAA	45.0	50.0	58.0	58.0	1385 bp	0.5	59.4
petA-chlI_p4	AGCAGAGCAACCGTTAAACA	GATCGCTTTGGTATGCATGC	45.0	50.0	58.0	58.0	1671 bp	0.5	59.4
petA-chlI_p5	ACTCAAGCAGGGCAAGTTAC	GCAAAAGCACATGCAGCATA	50.0	45.0	58.1	58.0	1541 bp	0.5	59.2