Hotspot

rpl16

LSC · 22960-24227 · 1268 bp

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Pi 0.026

MarkerSeek score 69.00

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1268
variable_sites: 76
pi: 0.026
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	GGATTGCGCCAAAAATTCGA	CAAGTACAAATTGCAGGGCG	45.0	50.0	58.0	58.0	2004-2021 bp	0.5	65.3
rpl16_p2	TGCGCCAAAAATTCGAGTTC	CAAGTACAAATTGCAGGGCG	45.0	50.0	58.0	58.0	2000-2017 bp	0.5	65.0
rpl16_p3	TTGGGTGCCTCAAAATTGGA	CAAGTACAAATTGCAGGGCG	45.0	50.0	57.9	58.0	2227-2242 bp	0.5	64.2
rpl16_p4	GGATTGCGCCAAAAATTCGA	GAAATTGCACGTGTCGAGTG	45.0	50.0	58.0	58.1	1987-1988 bp	0.333333	53.2
rpl16_p5	TGCGCCAAAAATTCGAGTTC	GAAATTGCACGTGTCGAGTG	45.0	50.0	58.0	58.1	1983-1984 bp	0.333333	52.9