Hotspot

petB

LSC · 16748-18086 · 1339 bp

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Pi 0.0107

MarkerSeek score 60.30

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1339
variable_sites: 32
pi: 0.0107
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	TTGGTAGTTCGATCGTGGAA	TCCTCCCCTTAGTGATAAATGA	45.0	40.9	56.6	55.7	1433-1455 bp	1.0	44.7
petB_p2	TTTGGTAGTTCGATCGTGGA	TCCTCCCCTTAGTGATAAATGA	45.0	40.9	56.6	55.7	1434-1456 bp	1.0	44.7
petB_p3	TGGTAGTTCGATCGTGGAAT	TCCTCCCCTTAGTGATAAATGA	45.0	40.9	56.4	55.7	1432-1454 bp	1.0	44.7
petB_p4	TTGGTAGTTCGATCGTGGAA	TTCCTCCCCTTAGTGATAAATGA	45.0	39.1	56.6	56.4	1434-1456 bp	1.0	44.7
petB_p5	TTTGGTAGTTCGATCGTGGA	TTCCTCCCCTTAGTGATAAATGA	45.0	39.1	56.6	56.4	1435-1457 bp	1.0	44.7