Hotspot

ycf2-trnI-CAU

IRa · 88970-89148 · 179 bp

Back to Teucrium

Pi 0.0562

MarkerSeek score 66.30

Primer available yes

Label ycf2-trnI-CAU

Feature Metadata

label_name: ycf2-trnI-CAU
feature_type: igs
region: IRa
length_bp: 179
variable_sites: 22
pi: 0.0562
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnI-CAU_p1	GTTGAACCCTGTGAATTGCG	GAACCTACGACTTCGCCAAT	50.0	50.0	57.9	58.0	354-439 bp	1.0	91.5
ycf2-trnI-CAU_p2	CCTGTGAATTGCGCGAAAAT	GTTGGGCGCTTTAACCATTC	45.0	50.0	58.0	58.0	327-407 bp	0.833333	80.0
ycf2-trnI-CAU_p3	CCTGTGAATTGCGCGAAAAT	ATTGAACCTACGACTTCGCC	45.0	50.0	58.0	58.0	355-435 bp	0.833333	79.8
ycf2-trnI-CAU_p4	CCTGTGAATTGCGCGAAAAT	GAACCTACGACTTCGCCAAT	45.0	50.0	58.0	58.0	352-432 bp	0.833333	79.8
ycf2-trnI-CAU_p5	CCTGTGAATTGCGCGAAAAT	TTCTACCCTTTCCCGGAAGT	45.0	50.0	58.0	58.0	715-795 bp	0.833333	78.6