Hotspot

trnI-CAU-ycf2

IRb · 27892-28070 · 179 bp

Back to Teucrium

Pi 0.0553

MarkerSeek score 66.20

Primer available yes

Label trnI-CAU-ycf2

Feature Metadata

label_name: trnI-CAU-ycf2
feature_type: igs
region: IRb
length_bp: 179
variable_sites: 22
pi: 0.0553
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-ycf2_p1	GAACCTACGACTTCGCCAAT	GTTGAACCCTGTGAATTGCG	50.0	50.0	58.0	57.9	354-439 bp	1.0	91.5
trnI-CAU-ycf2_p2	GTTGGGCGCTTTAACCATTC	CCTGTGAATTGCGCGAAAAT	50.0	45.0	58.0	58.0	327-407 bp	0.833333	80.0
trnI-CAU-ycf2_p3	ATTGAACCTACGACTTCGCC	CCTGTGAATTGCGCGAAAAT	50.0	45.0	58.0	58.0	355-435 bp	0.833333	79.8
trnI-CAU-ycf2_p4	GAACCTACGACTTCGCCAAT	CCTGTGAATTGCGCGAAAAT	50.0	45.0	58.0	58.0	352-432 bp	0.833333	79.8
trnI-CAU-ycf2_p5	TTCTACCCTTTCCCGGAAGT	CCTGTGAATTGCGCGAAAAT	50.0	45.0	58.0	58.0	715-795 bp	0.833333	78.6