Hotspot

rps12-clpP

LSC · 11748-11905 · 158 bp

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Pi 0.0411

MarkerSeek score 66.60

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 158
variable_sites: 15
pi: 0.0411
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	TGACCTTGGTAGTCCGTTCT	TACAAAGGACACGCAAACCA	50.0	45.0	58.0	58.0	681-717 bp	1.0	86.3
rps12-clpP_p2	TGACCTTGGTAGTCCGTTCT	TAGAAAGCAACGGAGCCATC	50.0	50.0	58.0	58.0	1050-1086 bp	1.0	85.7
rps12-clpP_p3	TGCACCAACGGAAACCATAA	TAGAAAGCAACGGAGCCATC	45.0	50.0	58.0	58.0	907-941 bp	1.0	85.7
rps12-clpP_p4	TTGCACCAACGGAAACCATA	TAGAAAGCAACGGAGCCATC	45.0	50.0	58.0	58.0	908-942 bp	1.0	85.7
rps12-clpP_p5	TGACCTTGGTAGTCCGTTCT	AAGCAACGGAGCCATCATAG	50.0	50.0	58.0	58.0	1046-1082 bp	1.0	85.6