Hotspot

rrn4.5-rrn23

Genome · 133449-133546 · 98 bp

Back to Tetrataenium

Pi 0.0204

MarkerSeek score 64.20

Primer available yes

Label rrn4.5-rrn23

Feature Metadata

label_name: rrn4.5-rrn23
feature_type: igs
region: Genome
length_bp: 98
variable_sites: 2
pi: 0.0204
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn4.5-rrn23_p1	TCACTGCACTTCCACTTGAC	AAGTAGTAAGCCCACCCCAA	50.0	50.0	58.0	58.2	194 bp	1.0	78.5
rrn4.5-rrn23_p2	CTGTTAGGATGCCTCAGCTG	AAGTAGTAAGCCCACCCCAA	55.0	50.0	58.0	58.2	220 bp	1.0	78.3
rrn4.5-rrn23_p3	GTCTGTTAGGATGCCTCAGC	AAGTAGTAAGCCCACCCCAA	55.0	50.0	58.1	58.2	222 bp	1.0	78.3
rrn4.5-rrn23_p4	TCACTGCACTTCCACTTGAC	TAGTAAGCCCACCCCAAGAT	50.0	50.0	58.0	57.7	191 bp	1.0	78.2
rrn4.5-rrn23_p5	CTGTTAGGATGCCTCAGCTG	TAGTAAGCCCACCCCAAGAT	55.0	50.0	58.0	57.7	217 bp	1.0	78.1