Hotspot

ndhF-rpl32

Genome · 114592-115581 · 990 bp

Back to Tetrataenium

Pi 0.0206

MarkerSeek score 60.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: Genome
length_bp: 990
variable_sites: 20
pi: 0.0206
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AAGCGGGTCGATCAAGTAAC	AATTCCAGGCTTTTGTCGGT	50.0	45.0	58.0	58.0	1956-1997 bp	1.0	82.8
ndhF-rpl32_p2	AAGCGGGTCGATCAAGTAAC	ATTCCAGGCTTTTGTCGGTT	50.0	45.0	58.0	58.0	1955-1996 bp	1.0	82.8
ndhF-rpl32_p3	GTTGCCGACGGAAAAAGAAG	ATTCCAGGCTTTTGTCGGTT	50.0	45.0	58.0	58.0	1777-1818 bp	1.0	82.8
ndhF-rpl32_p4	CCGTGTGAACCAAAACCCTA	AATTCCAGGCTTTTGTCGGT	50.0	45.0	58.0	58.0	2184-2225 bp	1.0	82.3
ndhF-rpl32_p5	CCGTGTGAACCAAAACCCTA	ATTCCAGGCTTTTGTCGGTT	50.0	45.0	58.0	58.0	2183-2224 bp	1.0	82.3