Hotspot

ycf1-ndhF

Genome · 112269-112353 · 85 bp

Back to Tetrataenium

Pi 0.45

MarkerSeek score 64.50

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: Genome
length_bp: 85
variable_sites: 9
pi: 0.45
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.24
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	ACATGCCGAAGTGATGGAAA	GTTGGATCCCTCGGAATTCC	45.0	55.0	57.8	58.0	1289-1374 bp	1.0	83.2
ycf1-ndhF_p2	ACATGCCGAAGTGATGGAAA	TGGATCCCTCGGAATTCCTT	45.0	50.0	57.8	58.1	1287-1372 bp	1.0	82.6
ycf1-ndhF_p3	ACATGCCGAAGTGATGGAAA	TTGGATCCCTCGGAATTCCT	45.0	50.0	57.8	58.1	1288-1373 bp	1.0	82.6
ycf1-ndhF_p4	CCCTGCCCACACTAGAAAAA	GTTGGATCCCTCGGAATTCC	50.0	55.0	57.7	58.0	1201-1286 bp	1.0	82.6
ycf1-ndhF_p5	TACGTATCCGCCTAGCTTGT	GTTGGATCCCTCGGAATTCC	50.0	55.0	58.3	58.0	1254-1339 bp	1.0	82.3