Hotspot

psbE-petL

Genome · 65082-66083 · 1002 bp

Back to Tetrataenium

Pi 0.0136

MarkerSeek score 67.10

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: Genome
length_bp: 1002
variable_sites: 11
pi: 0.0136
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GGTTACCGTTAAAGCCGCTA	50.0	50.0	57.9	58.0	969-1160 bp	1.0	84.3
psbE-petL_p2	ACTGACGAATAACCAACCCG	GGTTACCGTTAAAGCCGCTA	50.0	50.0	57.9	58.0	982-1173 bp	1.0	84.0
psbE-petL_p3	CAACCCGCAATGAATAGGGA	TGAGGTTACCGTTAAAGCCG	50.0	50.0	57.9	57.9	972-1163 bp	1.0	83.7
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	GGTTACCGTTAAAGCCGCTA	50.0	50.0	57.8	58.0	892-1083 bp	1.0	83.6
psbE-petL_p5	ACTGACGAATAACCAACCCG	TGAGGTTACCGTTAAAGCCG	50.0	50.0	57.9	57.9	985-1176 bp	1.0	83.4