primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	atpF-atpH	1	ATTAAACCCGAAACTCCCGG	GCCTGGTTGTAGCATTAGCA	20	20	50.0	50.0	57.9	58.2	0.0	0.0	0.368120	12118	12456	429-455	1.000000	82.0
atpF-atpH_p2	atpF-atpH	2	CCAGTGGCCTAAAGAAACGA	GCCTGGTTGTAGCATTAGCA	20	20	50.0	50.0	57.8	58.2	3.3	0.0	0.428719	12118	12456	402-428	1.000000	81.5
atpF-atpH_p3	atpF-atpH	3	ATTAAACCCGAAACTCCCGG	TGGCCTGGTTGTAGCATTAG	20	20	50.0	50.0	57.9	57.6	0.0	3.3	0.536681	12118	12456	431-457	1.000000	80.6
atpF-atpH_p4	atpF-atpH	4	ATTAAACCCGAAACTCCCGG	TGAGGCGGAGGGAAAAATAC	20	20	50.0	50.0	57.9	57.6	0.0	0.0	0.531623	12118	12456	500-526	1.000000	80.5
atpF-atpH_p5	atpF-atpH	5	CCAGTGGCCTAAAGAAACGA	TGAGGCGGAGGGAAAAATAC	20	20	50.0	50.0	57.8	57.6	3.3	0.0	0.592222	12118	12456	473-499	1.000000	80.1
rps2-rpoC2_p1	rps2-rpoC2	1	AATGAACTCCGGCCTTCATC	GCCTTTCAAACAATTTCCACG	20	21	50.0	42.9	58.0	56.9	20.5	0.0	2.171446	15585	15804	337-346	1.000000	66.4
rps2-rpoC2_p2	rps2-rpoC2	2	CCGGCCTTCATCATCTCTTC	GCCTTTCAAACAATTTCCACG	20	21	55.0	42.9	58.1	56.9	0.0	0.0	2.251001	15585	15804	329-338	1.000000	65.8
rps2-rpoC2_p3	rps2-rpoC2	3	CGGCCTTCATCATCTCTTCC	GCCTTTCAAACAATTTCCACG	20	21	55.0	42.9	58.1	56.9	0.0	0.0	2.251001	15585	15804	328-337	1.000000	65.8
rps2-rpoC2_p4	rps2-rpoC2	4	AATGAACTCCGGCCTTCATC	GCCTTTCAAACAATTTCCACGA	20	22	50.0	40.9	58.0	58.3	20.5	0.0	2.331936	15585	15804	337-346	1.000000	65.1
rps2-rpoC2_p5	rps2-rpoC2	5	CGGCCTTCATCATCTCTTCC	GCCTTTCAAACAATTTCCACGA	20	22	55.0	40.9	58.1	58.3	0.0	0.0	2.411491	15585	15804	328-337	1.000000	64.6
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.175803	30704	31475	874-876	1.000000	81.2
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	GTGTAGTACCCTACCCCCAG	GAACCGATGACTTACGCCTT	20	20	60.0	50.0	58.2	58.0	0.0	0.0	0.220324	30704	31475	909-911	1.000000	80.7
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	CGTTGCCTCCTTGAAAGAGA	GGCGTTACTCTACCGTTGAG	20	20	50.0	55.0	57.8	58.1	0.0	8.3	0.264383	30704	31475	849-851	1.000000	80.5
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	GTGTAGTACCCTACCCCCAG	GGCGTTACTCTACCGTTGAG	20	20	60.0	55.0	58.2	58.1	0.0	8.3	0.308904	30704	31475	884-886	1.000000	80.1
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	CGTTGCCTCCTTGAAAGAGA	CCGATGACTTACGCCTTACC	20	20	50.0	55.0	57.8	58.2	0.0	0.0	0.320736	30704	31475	871-873	1.000000	80.0
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	GGGATAGCTCAGCTGGTAGA	TGGATAGGATGGCCTTTACG	20	20	55.0	50.0	58.0	56.8	10.3	3.3	1.224095	48822	49218	536-543	1.000000	71.2
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	GGGATAGCTCAGCTGGTAGA	GGATAGGATGGCCTTTACGT	20	20	55.0	50.0	58.0	56.8	10.3	3.3	1.226313	48822	49218	535-542	1.000000	71.2
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACG	20	20	50.0	50.0	58.1	56.8	0.0	3.3	1.273485	48822	49218	500-507	1.000000	70.9
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	CCTCGTGTCACCAGTTCAAA	GGATAGGATGGCCTTTACGT	20	20	50.0	50.0	58.1	56.8	0.0	3.3	1.275704	48822	49218	499-506	1.000000	70.9
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	GGGATAGCTCAGCTGGTAGA	TGAAAGTTGGATAGGATGGCC	20	21	55.0	47.6	58.0	57.7	10.3	0.0	1.299640	48822	49218	543-550	1.000000	70.6
petA-psbJ_p1	petA-psbJ	1	CTTACGTGTCCAAGGCCTTT	GCTGGTATTCTTGTGATCGGT	20	21	50.0	47.6	58.1	58.1	17.6	0.0	1.201437	63348	64300	1143-1165	1.000000	72.7
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTGGC	GCTGGTATTCTTGTGATCGGT	20	21	50.0	47.6	57.8	58.1	0.0	0.0	1.248500	63348	64300	1130-1152	1.000000	72.4
petA-psbJ_p3	petA-psbJ	3	CTTACGTGTCCAAGGCCTTT	AGCTGGTATTCTTGTGATCGG	20	21	50.0	47.6	58.1	57.8	17.6	0.0	1.293037	63348	64300	1144-1166	1.000000	72.0
petA-psbJ_p4	petA-psbJ	4	GGCCTTTTGTTCTTCTTGGC	AGCTGGTATTCTTGTGATCGG	20	21	50.0	47.6	57.8	57.8	0.0	0.0	1.340100	63348	64300	1131-1153	1.000000	71.7
petA-psbJ_p5	petA-psbJ	5	GATCCCTTACGTGTCCAAGG	GCTGGTATTCTTGTGATCGGT	20	21	55.0	47.6	57.7	58.1	7.7	0.0	1.404770	63348	64300	1148-1170	1.000000	71.1
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	GGTTACCGTTAAAGCCGCTA	20	20	50.0	50.0	57.9	58.0	6.4	0.0	0.060086	65082	66083	969-1160	1.000000	84.3
psbE-petL_p2	psbE-petL	2	ACTGACGAATAACCAACCCG	GGTTACCGTTAAAGCCGCTA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.097701	65082	66083	982-1173	1.000000	84.0
psbE-petL_p3	psbE-petL	3	CAACCCGCAATGAATAGGGA	TGAGGTTACCGTTAAAGCCG	20	20	50.0	50.0	57.9	57.9	6.4	0.0	0.134501	65082	66083	972-1163	1.000000	83.7
psbE-petL_p4	psbE-petL	4	AGAACGTTCTCCTGTGCTTC	GGTTACCGTTAAAGCCGCTA	20	20	50.0	50.0	57.8	58.0	24.9	0.0	0.165095	65082	66083	892-1083	1.000000	83.6
psbE-petL_p5	psbE-petL	5	ACTGACGAATAACCAACCCG	TGAGGTTACCGTTAAAGCCG	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.172116	65082	66083	985-1176	1.000000	83.4
psbT-psbN_p1	psbT-psbN	1	GAGAACCGCCTAAAGTTCCA	TCGAGGAACATGGGGACTAG	20	20	50.0	55.0	57.5	58.2	0.0	0.0	0.690904	74507	74592	138-138	1.000000	76.2
psbT-psbN_p2	psbT-psbN	2	GAGAACCGCCTAAAGTTCCA	TTCGAGGAACATGGGGACTA	20	20	50.0	50.0	57.5	57.8	0.0	0.0	0.708847	74507	74592	139-139	1.000000	76.1
psbT-psbN_p3	psbT-psbN	3	GAGAACCGCCTAAAGTTCCA	GATCCCTTCGAGGAACATGG	20	20	50.0	55.0	57.5	57.8	0.0	0.0	0.714285	74507	74592	145-145	1.000000	75.9
psbT-psbN_p4	psbT-psbN	4	GAGAACCGCCTAAAGTTCCA	GGGTACGCCTTATATACCGC	20	20	50.0	55.0	57.5	57.7	0.0	0.0	0.751829	74507	74592	193-193	1.000000	75.2
psbT-psbN_p5	psbT-psbN	5	GAGAACCGCCTAAAGTTCCA	GTTTTACCGGGTACGCCTTA	20	20	50.0	50.0	57.5	57.6	0.0	6.3	0.846011	74507	74592	201-201	1.000000	74.4
trnH-GUG-rrn16_p1	trnH-GUG-rrn16	1	CGGATGTAGCCAAGTGGATC	CCGACTTGCATGTGTTAAGC	20	20	55.0	50.0	58.1	57.8	0.2	10.4	0.376231	100628	101832	1344-1684	1.000000	81.5
trnH-GUG-rrn16_p2	trnH-GUG-rrn16	2	TGGATCAAGGCAGTGGATTG	CCGACTTGCATGTGTTAAGC	20	20	50.0	50.0	57.9	57.8	0.0	10.4	0.380592	100628	101832	1330-1670	1.000000	81.5
trnH-GUG-rrn16_p3	trnH-GUG-rrn16	3	GGATCAAGGCAGTGGATTGT	CCGACTTGCATGTGTTAAGC	20	20	50.0	50.0	57.9	57.8	0.0	10.4	0.382100	100628	101832	1329-1669	1.000000	81.5
trnH-GUG-rrn16_p4	trnH-GUG-rrn16	4	GTGGATCAAGGCAGTGGATT	CCGACTTGCATGTGTTAAGC	20	20	50.0	50.0	57.9	57.8	0.0	10.4	0.382100	100628	101832	1331-1671	1.000000	81.5
trnH-GUG-rrn16_p5	trnH-GUG-rrn16	5	CGGATGTAGCCAAGTGGATC	GCAGGTTCTTACGCGTTACT	20	20	55.0	50.0	58.1	58.3	0.2	20.8	0.422477	100628	101832	1397-1737	1.000000	81.0
rrn23-rrn4.5_p1	rrn23-rrn4.5	1	AAGTAGTAAGCCCACCCCAA	TCACTGCACTTCCACTTGAC	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.296419	108555	108652	194-194	1.000000	78.5
rrn23-rrn4.5_p2	rrn23-rrn4.5	2	AAGTAGTAAGCCCACCCCAA	CTGTTAGGATGCCTCAGCTG	20	20	50.0	55.0	58.2	58.0	0.0	7.6	0.299150	108555	108652	220-220	1.000000	78.3
rrn23-rrn4.5_p3	rrn23-rrn4.5	3	AAGTAGTAAGCCCACCCCAA	GTCTGTTAGGATGCCTCAGC	20	20	50.0	55.0	58.2	58.1	0.0	0.0	0.303233	108555	108652	222-222	1.000000	78.3
rrn23-rrn4.5_p4	rrn23-rrn4.5	4	TAGTAAGCCCACCCCAAGAT	TCACTGCACTTCCACTTGAC	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.326749	108555	108652	191-191	1.000000	78.2
rrn23-rrn4.5_p5	rrn23-rrn4.5	5	TAGTAAGCCCACCCCAAGAT	CTGTTAGGATGCCTCAGCTG	20	20	50.0	55.0	57.7	58.0	0.0	7.6	0.329480	108555	108652	217-217	1.000000	78.1
ycf1-ndhF_p1	ycf1-ndhF	1	ACATGCCGAAGTGATGGAAA	GTTGGATCCCTCGGAATTCC	20	20	45.0	55.0	57.8	58.0	0.0	15.0	0.230123	112269	112353	1289-1374	1.000000	83.2
ycf1-ndhF_p2	ycf1-ndhF	2	ACATGCCGAAGTGATGGAAA	TGGATCCCTCGGAATTCCTT	20	20	45.0	50.0	57.8	58.1	0.0	22.1	0.305512	112269	112353	1287-1372	1.000000	82.6
ycf1-ndhF_p3	ycf1-ndhF	3	ACATGCCGAAGTGATGGAAA	TTGGATCCCTCGGAATTCCT	20	20	45.0	50.0	57.8	58.1	0.0	22.1	0.305512	112269	112353	1288-1373	1.000000	82.6
ycf1-ndhF_p4	ycf1-ndhF	4	CCCTGCCCACACTAGAAAAA	GTTGGATCCCTCGGAATTCC	20	20	50.0	55.0	57.7	58.0	0.0	15.0	0.317660	112269	112353	1201-1286	1.000000	82.6
ycf1-ndhF_p5	ycf1-ndhF	5	TACGTATCCGCCTAGCTTGT	GTTGGATCCCTCGGAATTCC	20	20	50.0	55.0	58.3	58.0	0.0	15.0	0.347352	112269	112353	1254-1339	1.000000	82.3
ndhF_p1	ndhF	1	TTGAGGCTCTAGATACGGGA	TTGAAAGGGGGTAGGATCCG	20	20	50.0	55.0	57.0	58.8	16.7	6.0	1.834845	112354	114591	2846-2922	1.000000	68.3
ndhF_p2	ndhF	2	TTGAGGCTCTAGATACGGGA	GGGGTAGGATCCGGTAACTC	20	20	50.0	60.0	57.0	58.7	16.7	6.0	1.708336	112354	114591	2915-2915	0.500000	46.3
ndhF_p3	ndhF	3	TGAGGCTCTAGATACGGGAT	GGGGTAGGATCCGGTAACTC	20	20	50.0	60.0	56.7	58.7	16.7	6.0	1.941806	112354	114591	2914-2914	0.500000	44.5
ndhF_p4	ndhF	4	AAATTGGAAGGGCCTCTCTT	GGGGTAGGATCCGGTAACTC	20	20	45.0	60.0	56.7	58.7	6.5	6.0	1.971850	112354	114591	2584-2584	0.500000	44.2
ndhF_p5	ndhF	5	AATTGGAAGGGCCTCTCTTT	GGGGTAGGATCCGGTAACTC	20	20	45.0	60.0	56.7	58.7	6.5	6.0	1.971850	112354	114591	2583-2583	0.500000	44.2
ndhF-rpl32_p1	ndhF-rpl32	1	AAGCGGGTCGATCAAGTAAC	AATTCCAGGCTTTTGTCGGT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.010594	114592	115581	1956-1997	1.000000	82.8
ndhF-rpl32_p2	ndhF-rpl32	2	AAGCGGGTCGATCAAGTAAC	ATTCCAGGCTTTTGTCGGTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.010594	114592	115581	1955-1996	1.000000	82.8
ndhF-rpl32_p3	ndhF-rpl32	3	GTTGCCGACGGAAAAAGAAG	ATTCCAGGCTTTTGTCGGTT	20	20	50.0	45.0	58.0	58.0	20.5	0.0	0.037038	114592	115581	1777-1818	1.000000	82.8
ndhF-rpl32_p4	ndhF-rpl32	4	CCGTGTGAACCAAAACCCTA	AATTCCAGGCTTTTGTCGGT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.034532	114592	115581	2184-2225	1.000000	82.3
ndhF-rpl32_p5	ndhF-rpl32	5	CCGTGTGAACCAAAACCCTA	ATTCCAGGCTTTTGTCGGTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.034532	114592	115581	2183-2224	1.000000	82.3
rrn4.5-rrn23_p1	rrn4.5-rrn23	1	TCACTGCACTTCCACTTGAC	AAGTAGTAAGCCCACCCCAA	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.296419	133449	133546	194-194	1.000000	78.5
rrn4.5-rrn23_p2	rrn4.5-rrn23	2	CTGTTAGGATGCCTCAGCTG	AAGTAGTAAGCCCACCCCAA	20	20	55.0	50.0	58.0	58.2	7.6	0.0	0.299150	133449	133546	220-220	1.000000	78.3
rrn4.5-rrn23_p3	rrn4.5-rrn23	3	GTCTGTTAGGATGCCTCAGC	AAGTAGTAAGCCCACCCCAA	20	20	55.0	50.0	58.1	58.2	0.0	0.0	0.303233	133449	133546	222-222	1.000000	78.3
rrn4.5-rrn23_p4	rrn4.5-rrn23	4	TCACTGCACTTCCACTTGAC	TAGTAAGCCCACCCCAAGAT	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.326749	133449	133546	191-191	1.000000	78.2
rrn4.5-rrn23_p5	rrn4.5-rrn23	5	CTGTTAGGATGCCTCAGCTG	TAGTAAGCCCACCCCAAGAT	20	20	55.0	50.0	58.0	57.7	7.6	0.0	0.329480	133449	133546	217-217	1.000000	78.1
rrn16-trnH-GUG_p1	rrn16-trnH-GUG	1	CCGACTTGCATGTGTTAAGC	GATTGTGAATCCACCATGCG	20	20	50.0	50.0	57.8	57.5	10.4	0.0	0.727535	140269	141473	1315-1315	0.500000	54.2
rrn16-trnH-GUG_p2	rrn16-trnH-GUG	2	GCAGGTTCTTACGCGTTACT	GATTGTGAATCCACCATGCG	20	20	50.0	50.0	58.3	57.5	20.8	0.0	0.773781	140269	141473	1368-1368	0.500000	53.8
rrn16-trnH-GUG_p3	rrn16-trnH-GUG	3	TTCTTACGCGTTACTCACCC	GATTGTGAATCCACCATGCG	20	20	50.0	50.0	57.6	57.5	20.8	0.0	0.832727	140269	141473	1363-1363	0.500000	53.3
rrn16-trnH-GUG_p4	rrn16-trnH-GUG	4	CCGACTTGCATGTGTTAAGC	TCAAGGCAGTGGATTGTGAA	20	20	50.0	45.0	57.8	57.3	10.4	0.0	0.902400	140269	141473	1326-1326	0.500000	52.8
rrn16-trnH-GUG_p5	rrn16-trnH-GUG	5	GCAGGTTCTTACGCGTTACT	TCAAGGCAGTGGATTGTGAA	20	20	50.0	45.0	58.3	57.3	20.8	0.0	0.948646	140269	141473	1379-1379	0.500000	52.4