Hotspot

ycf1

SSC · 128973-134612 · 5640 bp

Back to Tetrastigma

Pi 0.012

MarkerSeek score 54.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5640
variable_sites: 344
pi: 0.012
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CTCTCTTTTCGCCCTCTGTC	GAAAGATGCCCTTGTCCACA	55.0	50.0	58.0	58.1	1082-1094 bp	0.823529	79.2
ycf1_p2	CGGCTACTTTTATCCGTGCT	GAAAGATGCCCTTGTCCACA	50.0	50.0	58.1	58.1	1107-1119 bp	0.823529	78.7
ycf1_p3	TCGGCTACTTTTATCCGTGC	GAAAGATGCCCTTGTCCACA	50.0	50.0	58.1	58.1	1108-1120 bp	0.823529	78.6
ycf1_p4	CTCTGTCTTTTCTCCCCTGC	GAAAGATGCCCTTGTCCACA	55.0	50.0	57.9	58.1	1069-1081 bp	0.764706	75.0
ycf1_p5	GTTTCTGCAATTTCTCCGCC	GAAAGATGCCCTTGTCCACA	50.0	50.0	58.0	58.1	679-691 bp	0.529412	63.8