Hotspot

ndhF

SSC · 114682-116928 · 2247 bp

Back to Tetrastigma

Pi 0.0116

MarkerSeek score 56.90

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2247
variable_sites: 146
pi: 0.0116
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GAGGATCCGGACAAAATCGA	ACTTGACCGCAGTAGATCCT	50.0	50.0	57.4	58.1	2801-2949 bp	0.823529	75.4
ndhF_p2	GAGGATCCGGACAAAATCGAT	ACTTGACCGCAGTAGATCCT	47.6	50.0	57.9	58.1	2801-2949 bp	0.823529	71.3
ndhF_p3	AGGATCCGGACAAAATCGATG	ACTTGACCGCAGTAGATCCT	47.6	50.0	58.2	58.1	2800-2948 bp	0.823529	70.9
ndhF_p4	GGATCCGGACAAAATCGATGA	ACTTGACCGCAGTAGATCCT	47.6	50.0	58.2	58.1	2799-2947 bp	0.823529	70.9
ndhF_p5	ACCGAAGAAGATCCTTCTCCT	ACTTGACCGCAGTAGATCCT	47.6	50.0	57.9	58.1	2867-3000 bp	0.470588	55.8