Hotspot

ccsA-ndhD

SSC · 120384-120635 · 252 bp

Back to Tetrastigma

Pi 0.0311

MarkerSeek score 70.40

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 252
variable_sites: 26
pi: 0.0311
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	CAAATTCCGCAATTGTCGCT	GCATGAGCGGTTTTGTTTCA	45.0	45.0	58.0	57.9	712-739 bp	1.0	87.0
ccsA-ndhD_p2	TGTCGCTTCTATGGGCTTTC	GCATGAGCGGTTTTGTTTCA	50.0	45.0	58.0	57.9	699-726 bp	1.0	87.0
ccsA-ndhD_p3	CAAATTCCGCAATTGTCGCT	ATGCGGCTTCAACATCTTCT	45.0	45.0	58.0	57.9	1012-1039 bp	1.0	85.4
ccsA-ndhD_p4	TGTCGCTTCTATGGGCTTTC	ATGCGGCTTCAACATCTTCT	50.0	45.0	58.0	57.9	999-1026 bp	1.0	85.4
ccsA-ndhD_p5	TCCAAGTCACAAATTCCGCA	ATGCGGCTTCAACATCTTCT	45.0	45.0	58.0	57.9	1021-1048 bp	1.0	85.3