Hotspot

ndhF-rpl32

SSC · 116929-118057 · 1129 bp

Back to Tetrastigma

Pi 0.0209

MarkerSeek score 78.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1129
variable_sites: 123
pi: 0.0209
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAGAAGTCCCACTCCTAGT	CCACCATCCCTTCCTTTTCC	55.0	55.0	57.5	58.1	1284-1425 bp	1.0	86.2
ndhF-rpl32_p2	GGAGAAGTCCCACTCCTAGT	CCCACCATCCCTTCCTTTTC	55.0	55.0	57.5	58.1	1285-1426 bp	1.0	86.2
ndhF-rpl32_p3	GGAGAAGTCCCACTCCTAGT	AACGCAAAAGCCTTTAACGC	55.0	45.0	57.5	58.5	1307-1448 bp	1.0	83.1
ndhF-rpl32_p4	GGAGAAGTCCCACTCCTAGT	CACCATCCCTTCCTTTTCCA	55.0	50.0	57.5	57.4	1283-1424 bp	1.0	82.6
ndhF-rpl32_p5	TGGAAAAAGGAGAAGTCCCAC	CCACCATCCCTTCCTTTTCC	47.6	55.0	57.8	58.1	1292-1433 bp	0.941176	77.9