Hotspot

rpl20-rps12

LSC · 73061-73832 · 772 bp

Back to Tetrastigma

Pi 0.0129

MarkerSeek score 74.70

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 772
variable_sites: 46
pi: 0.0129
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CTTGAATGGGCTCCTCGAAA	CGCCGAGGAACATGTACTAG	50.0	55.0	57.9	57.9	864-897 bp	1.0	86.3
rpl20-rps12_p2	TTGAATGGGCTCCTCGAAAG	CGCCGAGGAACATGTACTAG	50.0	55.0	57.9	57.9	863-896 bp	1.0	86.3
rpl20-rps12_p3	GCTCCTCGAAAGCTTGATGT	CGCCGAGGAACATGTACTAG	50.0	55.0	58.3	57.9	855-888 bp	1.0	85.1
rpl20-rps12_p4	CTTGAATGGGCTCCTCGAAA	GCCGAGGAACATGTACTAGG	50.0	55.0	57.9	57.5	863-896 bp	1.0	83.2
rpl20-rps12_p5	TTGAATGGGCTCCTCGAAAG	GCCGAGGAACATGTACTAGG	50.0	55.0	57.9	57.5	862-895 bp	1.0	83.2