Hotspot

psbC-trnS-UGA

LSC · 38552-38810 · 259 bp

Back to Tetrastigma

Pi 0.0309

MarkerSeek score 72.30

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 259
variable_sites: 26
pi: 0.0309
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	TCGTAGGTCATTTGTGGCAC	GGGTTCGAATCCCTCTCTCT	50.0	55.0	58.2	58.2	371-415 bp	1.0	86.1
psbC-trnS-UGA_p2	AGCTGCAGCAGGATTTGAAA	GGGTTCGAATCCCTCTCTCT	45.0	55.0	58.4	58.2	334-378 bp	1.0	85.0
psbC-trnS-UGA_p3	GCTGCAGCAGGATTTGAAAA	GGGTTCGAATCCCTCTCTCT	45.0	55.0	57.6	58.2	333-377 bp	1.0	84.5
psbC-trnS-UGA_p4	TTCGTAGGTCATTTGTGGCA	GGGTTCGAATCCCTCTCTCT	45.0	55.0	57.4	58.2	372-416 bp	1.0	83.3
psbC-trnS-UGA_p5	ATTCGTAGGTCATTTGTGGCA	GGGTTCGAATCCCTCTCTCT	42.9	55.0	57.9	58.2	373-417 bp	1.0	79.1