Hotspot

trnT-GGU-psbD

LSC · 34903-36120 · 1218 bp

Back to Tetrastigma

Pi 0.0109

MarkerSeek score 72.60

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1218
variable_sites: 75
pi: 0.0109
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	TCGCTTTCGTCTTTGGTGAA	55.0	45.0	58.1	58.1	1302-1357 bp	1.0	87.6
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	TCGCTTTCGTCTTTGGTGAA	50.0	45.0	58.1	58.1	1305-1360 bp	1.0	87.4
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	ATCGCTTTCGTCTTTGGTGA	55.0	45.0	58.1	57.8	1303-1358 bp	1.0	86.7
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	ATCGCTTTCGTCTTTGGTGA	50.0	45.0	58.1	57.8	1306-1361 bp	1.0	86.5
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1353-1408 bp	1.0	85.9