Hotspot

trnD-GUC-trnY-GUA

LSC · 33255-33713 · 459 bp

Back to Tetrastigma

Pi 0.0186

MarkerSeek score 70.80

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 459
variable_sites: 45
pi: 0.0186
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	CGGTGCTCTGACCAATTGAA	ATTCTTCTTGGGTCGATGCC	50.0	50.0	58.5	58.0	569-586 bp	1.0	86.0
trnD-GUC-trnY-GUA_p2	CGGTGCTCTGACCAATTGAA	TGGCAATATGTCTACGCTGG	50.0	50.0	58.5	57.8	516-533 bp	1.0	85.1
trnD-GUC-trnY-GUA_p3	CGGTGCTCTGACCAATTGAA	GGCAATATGTCTACGCTGGT	50.0	50.0	58.5	57.8	515-532 bp	1.0	85.1
trnD-GUC-trnY-GUA_p4	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.5	58.4	537-554 bp	1.0	83.9
trnD-GUC-trnY-GUA_p5	CGGTGCTCTGACCAATTGAA	GGGACGGACTGTAAATTCGT	50.0	50.0	58.5	57.6	536-553 bp	1.0	83.8