Hotspot

trnK-UUU-rps16

LSC · 4286-4977 · 692 bp

Back to Tetrastigma

Pi 0.0164

MarkerSeek score 68.90

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 692
variable_sites: 58
pi: 0.0164
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	AGGTTTTTACGGAACTTCGTCT	50.0	40.9	58.2	57.9	721-781 bp	0.882353	67.0
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	AGGTTTTTACGGAACTTCGTCT	50.0	40.9	58.2	57.9	720-780 bp	0.882353	67.0
trnK-UUU-rps16_p3	CAGTCGCGGTCTTACAAACT	AGGTTTTTACGGAACTTCGTCT	50.0	40.9	58.2	57.9	805-865 bp	0.882353	66.1
trnK-UUU-rps16_p4	TCAGTCGCGGTCTTACAAAC	AGGTTTTTACGGAACTTCGTCT	50.0	40.9	58.2	57.9	806-866 bp	0.882353	66.1
trnK-UUU-rps16_p5	TCTAGCCGCACTTAAAAGCC	AGGTTTTTACGGAACTTCGTCT	50.0	40.9	58.3	57.9	735-795 bp	0.882353	66.1