Hotspot

psbA-trnK-UUU

LSC · 1465-1721 · 257 bp

Back to Tetrastigma

Pi 0.0164

MarkerSeek score 69.40

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 257
variable_sites: 21
pi: 0.0164
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	ACGGTTTTCAGTGCTGGTTA	CTATCCGACTAGTTCCGGGT	45.0	55.0	57.7	58.0	376-410 bp	1.0	85.1
psbA-trnK-UUU_p2	ACGGTTTTCAGTGCTGGTTA	TCTATCCGACTAGTTCCGGG	45.0	55.0	57.7	57.7	377-411 bp	1.0	83.1
psbA-trnK-UUU_p3	AAGACGGTTTTCAGTGCTGG	CTATCCGACTAGTTCCGGGT	50.0	55.0	58.7	58.0	379-413 bp	1.0	82.2
psbA-trnK-UUU_p4	TACAGAAGCGACCCCATAGG	CTATCCGACTAGTTCCGGGT	55.0	55.0	58.6	58.0	350-384 bp	0.941176	80.4
psbA-trnK-UUU_p5	ATCCAGTTACAGAAGCGACC	CTATCCGACTAGTTCCGGGT	50.0	55.0	57.3	58.0	357-391 bp	0.941176	79.4