Hotspot

ycf4

LSC · 33868-34464 · 597 bp

Back to Tetraselmis

Pi 0.237

MarkerSeek score 81.20

Primer available yes

Label ycf4

Feature Metadata

label_name: ycf4
feature_type: gene
region: LSC
length_bp: 597
variable_sites: 138
pi: 0.237
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4_p1	GGGGTGAATGAAAACCGACA	CTAGATTGTGGTCGCGACAA	50.0	50.0	58.4	58.0	1841 bp	0.5	56.8
ycf4_p2	TGGGGTGAATGAAAACCGAC	CTAGATTGTGGTCGCGACAA	50.0	50.0	58.4	58.0	1842 bp	0.5	56.8
ycf4_p3	GGGGTGAATGAAAACCGACA	GTGAAGGTGGACGAACTGTT	50.0	50.0	58.4	58.1	1947 bp	0.5	56.5
ycf4_p4	TGGGGTGAATGAAAACCGAC	GTGAAGGTGGACGAACTGTT	50.0	50.0	58.4	58.1	1948 bp	0.5	56.5
ycf4_p5	GGGGTGAATGAAAACCGACA	TGAAGGTGGACGAACTGTTG	50.0	50.0	58.4	58.1	1946 bp	0.5	56.5