Hotspot

tilS

LSC · 40179-41327 · 1149 bp

Back to Tetraselmis

Pi 0.427

MarkerSeek score 81.90

Primer available yes

Label tilS

Feature Metadata

label_name: tilS
feature_type: gene
region: LSC
length_bp: 1149
variable_sites: 433
pi: 0.427
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tilS_p1	TCAGTTGGTAGAGCAGTGGA	ACAAGTGCACGTACTGTTGT	50.0	45.0	58.0	58.0	1679 bp	0.5	59.8
tilS_p2	TGGTAGAGCAGTGGACTGAA	ACAAGTGCACGTACTGTTGT	50.0	45.0	58.0	58.0	1674 bp	0.5	59.8
tilS_p3	TTGATAGCCGGGATAGCTCA	ACAAGTGCACGTACTGTTGT	50.0	45.0	58.0	58.0	1696 bp	0.5	59.8
tilS_p4	TTGGTAGAGCAGTGGACTGA	ACAAGTGCACGTACTGTTGT	50.0	45.0	58.0	58.0	1675 bp	0.5	59.8
tilS_p5	AGTTGGTAGAGCAGTGGACT	ACAAGTGCACGTACTGTTGT	50.0	45.0	58.0	58.0	1677 bp	0.5	59.7